人環(huán)指蛋白39(RNF39) ELISA試劑盒簡(jiǎn)單介紹：

實(shí)驗方法：酶聯(lián)免疫法/酶免法（ELISA）

檢測用途：科研實(shí)驗等領(lǐng)域科學(xué)研究，不得用于臨床診斷

保存條件：2~8℃，保質(zhì)期為六個(gè)月

產(chǎn)品應用：被測樣本中定性定量分析

產(chǎn)品規格：96T/盒可檢測90個(gè)樣本、48T/盒可檢測42個(gè)樣本

樣本類(lèi)型：標本必須為液體不含沉淀。包括像血清、血漿、組織勻漿、細胞培養上清液、尿液、胸腹水、腦脊液等。取材前須向銷(xiāo)售人員所要說(shuō)明書(shū)。

人環(huán)指蛋白39(RNF39) ELISA試劑盒試劑準備： 

1、使用前，所有的組分都要至少復溫 120min，確保充分復溫到室溫。 

2、濃縮洗滌液：從冰箱取出的濃縮洗滌液，會(huì )有結晶產(chǎn)生，這屬于正常現象，水浴加熱使結 晶完全溶解。濃縮洗滌液與蒸餾水，按 1:20 稀釋?zhuān)?1 份的濃縮洗滌液，添加 19 份的蒸 餾水。 

3、底物：底物液 A 和 B，在使用前，按 1:1 體積充分混合，混合后 15 分鐘內使用。

ELISA試劑盒常見(jiàn)問(wèn)題解答：

1.問(wèn)：我的標準曲線(xiàn)看起來(lái)很好，但我沒(méi)有在樣本中得到預期的信號，這是為什么？答：樣品可能不含分析物。矩陣效應可能掩蓋檢測。確保按照我司試劑盒中說(shuō)明書(shū)進(jìn)行稀釋。稀釋?zhuān)罢垯z查以確保稀釋操作正確。過(guò)度稀釋可能導致樣品低于標準曲線(xiàn)的范圍。Q:My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?A:The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

2.問(wèn)：你建議我該如何洗板？答：如果您使用的是自動(dòng)洗板機，我們建議定期檢查校正，并在清洗前用洗板機緩沖液沖洗系統。手動(dòng)洗板機也是如此。或者使用中繼器或洗滌瓶。用戶(hù)應小心確保所有內容物都被吸入，并在無(wú)棉紙上輕敲干燥。Q:How do you recommend I wash my plate?A:If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, and that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated and the plate tapped dry on lint-free paper.